How Many Cells Can A Cell Style Be Applied To Flow Cytometry – Flow Cell Care and Maintenance

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Flow Cytometry – Flow Cell Care and Maintenance

Improper use and maintenance of flow cytometry can lead to inaccurate readings and inaccurate data. Improper maintenance can also mean having to spend hours on the phone with customer service, having to send your machine in for service, or having to replace the entire cytometer cell. Sometimes the entire optics system must be removed to replace the cell.

Drugs to stay away from

Today’s business model is that most cytometry cells are made of quartz windows or fused silica components, so staying away from alkaline solutions with a pH greater than 9.5 is important. These solutions can attack the quartz and make it look bad. What happens is that these solutions can disrupt the quartz making the flow channel of the cell almost unusable. Users will see “streaks” in the channels of the flow of the cell and if the streaks appear where the laser or detector is, the phone will not work.

How to avoid crystallization

Avoid crystallization is very important. To avoid crystallization in the flow chamber, the user must always rinse it with water. Must be rinsed thoroughly after running buffers or salt solutions, especially high pH. Whenever researchers leave a device unused during the night, it is important to make sure that the drains have at least 10% organic mobile phase to prevent algae growth. Special coatings can be applied to quartz products to help prevent these types of growth.

Washing

Clean aspirators and optical equipment/assemblies are essential to the proper operation of any flow cytometer or any machine that uses cell phones. An important warning is to not allow the solution to dry in the flow channel of the cell. Solutions that contain dissolved salts, proteins or other solvents will block the flow. Also researchers should be careful not to let things into the cell phone as damage to the cell phone, such as scratches, will occur. Scratches will have a negative impact on the operation of the cells and if they are in the catchment area, the cells will not work.

For easy cleaning, rinse a small amount of distilled water from the cytometry cell. Use a syringe and fill it with a 10% detergent active solution. Insert the syringe into the flow channel and spray the detergent into the cell. After a few squirts, leave the solution in the flow chamber for a little over 2 hours. The flow cell should be rinsed thoroughly with distilled water.

One night a cell can be left with no. Weeks and long-term storage NSG recommends rinsing the cell with distilled water after filling it with 20% ethanol. For drying the flowing water can be dried using a stream of pure nitrogen.

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